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Addgene inc mettl20
Mettl20, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mettl20/pF714-pET28a-Hs-FL-METTL20-NHis+wt+(Plasmid+%2385115)/pm37703462__ja3c07299_si_001-146-42-43
Average 91 stars, based on 1 article reviews
mettl20 - by Bioz Stars, 2026-11
91/100 stars

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Related Articles

Purification:

Article Title: Activity Guided Azide-methyllysine Photo-trapping for Substrate Profiling of Lysine Demethylases.
Article Snippet: .. Catalytic domains of G9a and Suv39H2 and chromodomains of CBX1 were expressed and purified as reported previously.2 The following N-terminal 6xHis-tagged lysine methyltransferase bacterial S9 expression constructs in pET28a or pET 28-MHL were obtained from Addgene: CAMKMT (Addgene #58761), DOT1L (Addgene #36196), METTL20 (Addgene #85115), METTL21A (Addgene #60091) and METTL21C (Addgene #60100). .. Plasmids were transformed into E. Coli BL21 (DE3) competent cells (Invitrogen) using appropriate resistant antibiotic.

Expressing:

Article Title: Activity Guided Azide-methyllysine Photo-trapping for Substrate Profiling of Lysine Demethylases.
Article Snippet: .. Catalytic domains of G9a and Suv39H2 and chromodomains of CBX1 were expressed and purified as reported previously.2 The following N-terminal 6xHis-tagged lysine methyltransferase bacterial S9 expression constructs in pET28a or pET 28-MHL were obtained from Addgene: CAMKMT (Addgene #58761), DOT1L (Addgene #36196), METTL20 (Addgene #85115), METTL21A (Addgene #60091) and METTL21C (Addgene #60100). .. Plasmids were transformed into E. Coli BL21 (DE3) competent cells (Invitrogen) using appropriate resistant antibiotic.

Construct:

Article Title: Activity Guided Azide-methyllysine Photo-trapping for Substrate Profiling of Lysine Demethylases.
Article Snippet: .. Catalytic domains of G9a and Suv39H2 and chromodomains of CBX1 were expressed and purified as reported previously.2 The following N-terminal 6xHis-tagged lysine methyltransferase bacterial S9 expression constructs in pET28a or pET 28-MHL were obtained from Addgene: CAMKMT (Addgene #58761), DOT1L (Addgene #36196), METTL20 (Addgene #85115), METTL21A (Addgene #60091) and METTL21C (Addgene #60100). .. Plasmids were transformed into E. Coli BL21 (DE3) competent cells (Invitrogen) using appropriate resistant antibiotic.



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